SESRIC organised a five days training course on “The Use of Advanced Technology in Cotton Production” within the framework of the OIC Cotton Capacity Building Programme (OIC-CTP) in Lahore and Multan, Pakistan on 12-16 of December 2016.
The training course mainly focused on the following topics:
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Genetic modification of cotton
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Competent cells preparation.
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Agrobacterium inoculum preparation and electroporation
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Embryo isolation and treatment.
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Demonstration about biolistic gun
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Culturing of treated embryo on MS medium.
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Sub-culturing of developing embryo on selection medium
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DNA isolation from transgenic crops.
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Gel interpretation of isolated DNA
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Introduction to polymerase chain reaction, types and its use in GMO detection
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PCR for amplification of gene of interest
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Gel interpretation of amplified product
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mRNA expression of transgene through real time PCR
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Setting up of real time PCR for virus quantification
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Interpretation of results of real time PCR
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Development of Bt Cotton
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Role of biosafety studies in the commercialization of transgenic cotton
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Short hairpin RNA engineering and in planta gene silencing
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Quantification of Bt protein in transgenic cotton
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Hybrid seed production technology, transformation and expression studies of Cry1Ac+Cry2A and GT gene
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Fluorescent in situ hybridization of transgenic cotton for copy
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Expression analysis of cotton plants through microarray
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Cotton crop production and management
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Cotton varietal evaluation and testing system.
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